Techniques 

In order to understand human disease at a molecular level, we are attempting to determine the molecular structures of the key complexes involved in disease processes. To do this we take advantage of both x-ray crystallography and electron cryo-microscopy. Protein structure determination remains an extremely difficult process, frequently taking many years and fraught with difficulty, however great strides have been made recently, particularly in the field of electron cryo-microscopy, resulting in the award of this year’s Nobel Prize for Chemistry. The section remains at the forefront of the structural biology community, both in the UK, and worldwide.

 
 

Protocols

Cryo-EM sample preparation is an arduous and sometimes frustrating process. We have developed new protocols to work with difficult proteins.

 

Software

Given that cryo-EM remains a relatively immature field, we have developed our own software to address specific problems that came up during our work.